Histone H3.3 (phospho Ser31) rabbit pAb Antibody

SKU: orb768592

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Description

Rabbit polyclonal antibody to Histone H3.3 (phospho-Ser31)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC
Dilution range	Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. AA range:16-65
Molecular Weight	15kD
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-H3F3A antibody, anti-H3.3A antibody, anti-H3F3 antibody, anti-PP781 antibody, anti-H3F3B antibody, anti-H3.3B antibody, anti-Histone H3.3 antibody

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Histone H3.3 (phospho Ser31) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H3.3 (Phospho-Ser31) Antibody.

Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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