HLA-DQ mAb (SPV-L3), InVivoPure+

SKU: orb3127240

Description

HLA-DQ monoclonal antibody (clone SPV-L3) is a mouse IgG2aκ antibody that specifically recognizes the human HLA-DQ molecule, a class II major histocompatibility complex (MHC) protein. HLA-DQ is a heterodimer composed of an alpha (DQA1) and beta (DQB1) chain, both anchored in the membrane of antigen-presenting cells (APCs) such as B cells, dendritic cells, and macrophages. SPV-L3 was raised against a T4-positive cytotoxic T lymphocyte clone (HG-38) and binds a monomorphic determinant of HLA-DQ. It is widely used in flow cytometry, immunocytochemistry, immunofluorescence, and immunohistochemistry, with validated reactivity in human and porcine samples. Importantly, SPV-L3 has been shown to block cytotoxic activity of T4-positive CTL clones, making it useful in functional assays and immune regulation studies. This antibody is often used in transplantation research, autoimmune disease studies, and immunopeptidomics, where HLA-DQ plays a role in antigen presentation and immune recognition.

Images & Validation

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Tested Applications	ELISA, FC, WB
Reactivity	Human
Application Notes	Recomended Isotype: Mouse IgG2A k Isotype Control, Recomended Isotype Catalog: orb2568793

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, Kappa
Clone No.	SPV-L3
Immunogen	T4-positive CTL clone HG-38
Target	HLA-DQ
Purity	≥ 95 % (CGE, reducing conditions) ≤ 5% aggregates (analytical SEC) Endotoxin level ≤ 1 EU/mg
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Concentration	≥ 1 mg/ mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, Human Leukocyte Antigen DQ, Anti-Human HLA-DQ, DC-alpha, DQ-A1, DC-1 alpha chain, Leukocyte antigen alpha chain, MHC class II DQA1, HLA-DCA, HLA-DQA, MHC class II antigen, CELIAC1, Leucocyte antigen DQA1, MHC class II HLA-DQ-alpha-1, MHC HLA-DQ alpha

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