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HLA-DRa antibody

SKU: orb2276407

Description

HLA-DRa antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Cell Biology

Images & Validation

Tested ApplicationsIHC
Dilution Range1:100-1:200
ReactivityHuman
Application Notes
Positive Control: Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in small capillaries of glomeruli. Negative Control: Absence of HLA-DRa staining is expected in hepatocytes of a completely normal liver and in tubuli of completely normal kidney. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

Key Properties

HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.MSVA-470R
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DR alpha chain; DRB1; DRB4; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1/3/4/5

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UniProt Details

No UniProt data available

Protocol Information

IHC
Immunohistochemistry
View Protocol

Available Sizes

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0.1 ml
$ 520.00
0.5 ml
$ 950.00
1 ml
$ 1,530.00
Free Secondary Antibody (20 ul)0/0

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