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HMGB1 antibody
SKU: orb195321
Featured
Description
Images & Validation
−Item 1 of 16
| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:5000, IF/ICC: 1:1000, IHC-P: 1:1000 |
| Reactivity | Bovine, Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human HMGB1. Please contact us for the exact immunogen sequence. The peptide is available as orb374832. |
| Target | HMGB1 |
| Molecular Weight | 24 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−anti Amphoterin antibody, anti Chromosomal protein, nonhistone, HMG1 antibody, anti High mobility group 1 antibody, anti High mobility group box 1 antibody, anti High mobility group protein 1 antibody, anti High mobility group protein B1 antibody, anti high-mobility group (nonhistone chromosomal) protein 1 antibody, anti HMG 1 antibody, anti HMG-1 antibody, anti HMG1 antibody, anti HMG3 antibody, anti HMGB-1 antibody, anti HMGB 1 antibody, anti HMGB1 antibody, anti Hmgb1 protein antibody, anti Nonhistone chromosomal protein HMG1 antibody, anti SBP 1 antibody, anti Sulfoglucuronyl carbohydrate binding protein antibody
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
WB analysis of rat kidney (Lane 1), brain (Lane 2) using HMGB1 antibody (Dilution at 1:5000)
Immunofluorescence analysis of mouse sp20 mouse myeloma cells tissue using anti-HMGB1 (dilution of primary antibody - 1:300)
IHC-P staining of mouse skin tissue using HMGB1 antibody (dilution at 1:300)
Immunofluorescence image of mouse sp23 mouse myeloma cells tissue using anti-HMGB1 (dilution at 1:300)
Immunofluorescence image of mouse sp22 mouse myeloma cells tissue using HMGB1 antibody (dilution at 1:1000)
IF image of rat skin tissue using HMGB1 antibody (primary antibody at 1:1000)
Immunofluorescence image of rat skin tissue using anti-HMGB1 (dilution at 1:1000)
Immunofluorescence image of mouse sp22 mouse myeloma cells tissue using anti-HMGB1 (dilution at 1:300)
IHC-P staining of rat skin tissue using anti-HMGB1 (dilution at 1:1000)
IF analysis of mouse sp21 mouse myeloma cells tissue using HMGB1 antibody (dilution of primary antibody at 1:300)
Immunofluorescence analysis of rat skin tissue using HMGB1 antibody (dilution of primary antibody - 1:1000)
Immunohistochemical staining of paraffin embedded mouse brain tissue using HMGB1 antibody (primary antibody at 1:1000)
Immunofluorescence analysis of mouse sp21 mouse myeloma cells tissue using anti-HMGB1 (dilution of primary antibody - 1:1000)
IF image of rat skin tissue using anti-HMGB1 (primary antibody at 1:1000)
Immunohistochemical staining of rat skin tissue using HMGB1 antibody (dilution of primary antibody - 1:300)
Western blot analysis of rat kidney (Lane 1), rat brain (Lane 2) using HMGB1 antibody (dilution at 1:200)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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F Li, N Qin, Y Yu, R Dong, X Li, S Gong, Z Zeng, L Huang, H Yang TREM-1 inhibition or ondansetron administration ameliorates NLRP3 inflammasome and pyroptosis in traumatic brain injury-induced acute lung injury Intensive Care Medicine, (2024)
Applications
WB
Reactivity
Rat
HMGB1 antibody (orb195321)
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