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HMGCS1 Antibody (C-term)
SKU: orb36825
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Hamster, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB20147 |
| Immunogen | Synthetic Peptide |
| Target | HMGCS1 |
| Molecular Weight | 57294 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Hydroxymethylglutaryl-CoA synthase antibody, anti-cytoplasmic antibody, anti-HMG-CoA synthase antibody, anti-3-hydroxy-3-methylglutaryl coenzyme A synthase antibody, anti-HMGCS antibody, anti-HMGCS1 antibody
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Western blot analysis of HMGCS1 antibody (C-term) in K562 cell line lysates (35 ug/lane). NKRF (arrow) was detected using the purified Pab.
Flow cytometric analysis of hela cells using HMGCS1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002121.4, NP_001091742.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
Jonckheere, Veronique et al. Omics Assisted N-terminal Proteoform and Protein Expression Profiling On Methionine Aminopeptidase 1 (MetAP1) Deletion Mol Cell Proteomics, 17, 694-708 (2018)
HMGCS1 Antibody (C-term) (orb36825)
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