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HOE 33187

SKU: orb1308225

Description

Hoechst stains are cell-permeable blue fluorescent dyes that bind to DNA, enabling the visualization of genetic material in both live and fixed cells. They are widely used in cell biology and biomedical research for applications such as chromosome staining, cell cycle analysis, and viability assays in vitro and in vivo.

Images & Validation

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HOE 33187

Key Properties

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CAS Number23623-08-7
MW408.5
Purity99.86%
FormulaC25H24N6
SMILESCN1CCN(CC1)c1ccc2nc([nH]c2c1)-c1ccc2nc([nH]c2c1)-c1ccccc1
TargetOthers
SolubilityDMSO:2 mg/mL (4.9 mM)

Bioactivity

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Cell Research
I. Nuclear staining 1. Material preparation: (1) Hoechst 33342 solution: Dissolve Hoechst 33342 in an appropriate solvent, such as water or PBS, with a common concentration of 1-10 µg/mL. (2) Cell Samples: Live cells or fixed cells, placed on a slide or in culture. (3) PBS buffer: Used to wash cells and adjust pH. 2. Experimental steps: Live cell staining 1) Add Hoechst 33342 to the cell culture medium to reac the recommended final concentration 2) Incubate at room temperature or 37°C for 15-30 minutes, depending o the cell type. 3) Was the cells with PBS buffer to remove excess dye. 4) Analyz the stained cells using a fluorescence microscope or flow cytometer. Excitation wavelength: 350 nM Emission wavelength: 461 nM Fixed cell staining: 1) Fi the cells using an appropriate fixative (such as 4% paraformaldehyde). 2) After fixation, stai the cells as described above, either on a slide or in suspension. 3) Analyz the fluorescence o the cells using a microscope. II. Application of Cell Cycle Analysis 1. Stain apoptotic cells as described above. 2. Examin the cells for morphological changes, such as chromatin condensation and nuclear fragmentation, which are hallmarks of apoptosis.
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Storage & Handling

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Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Inhibitor, inhibit, HOE33187, HOE-33187, HOE 33187

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Compound Identifiers

PubChem CID
44386667 ↗

Key Properties

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No computed properties available.

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HOE 33187 (orb1308225)

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Available Sizes

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5 mg
¥ 910.00
10 mg
¥ 1,040.00
25 mg
¥ 1,300.00
50 mg
¥ 1,950.00
100 mg
¥ 2,990.00
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