Hoechst 33258 analog 3

SKU: orb1307182

Description

Research Area

Cell Biology

Images & Validation

−

Item 1 of 1

Key Properties

−

CAS Number	23554-98-5
MW	422.52
Purity	99.4%
Formula	C₂₆H₂₆N₆
SMILES	CN1CCN(CC1)c1ccc2nc([nH]c2c1)-c1ccc2nc([nH]c2c1)-c1ccc(C)cc1
Target	Autophagy,Others
Solubility	DMSO:3.57 mg/mL (8.45 mM)

Bioactivity

−

In Vitro

The dyes Hoechst 33258 and Hoechst 33342 ar the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nM, and both emit blue/cyan fluorescent light around an emission maximum at 461 nM. Unbound dye has its maximum fluorescence emission in the 510-540 nM range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. concentration can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least Six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C . Althoug the dyes can bind to All nucleic acids, AT-rich double-st anded DNA st and enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells.

Cell Research

Instructions 1. Dissolution and dilution: Hoechst 33258 Analog 3 is usually dissolved in DMSO (if applicable) or PBS. Before use the dye can be diluted according to the concentration recommended b the manufacturer the generally recommended staining concentration is 10ug/ml, an the specific concentration needs to be optimized according to the Experimental requirements. 2. Staining steps: a. Fixation: If cells need to be fixed (for example, with formaldehyde or ice-cold methanol), fix them first. b. Staining: Add an appropriate amount of Hoechst 33258 Analog 3 solution to the cell culture plate and stain for about 15-30 minutes at room temperature. c. Rinse: Gently was the cells with PBS or an appropriate buffer to remove unbound dye. 3. Fluorescence microscopy the stained cells can be observed under ultraviolet light. Hoechst 33258 Analog 3 emits blue fluorescence, usually with an excitation light of 360-380 nM and an emission light in the range of 450-470 nM.

Storage & Handling

−

Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Hoechst 33258 analog 3, Inhibitor, inhibit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Chemical structure of Hoechst 33258 analog 3

Compound Identifiers

PubChem CID

10070883 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

CoA

Certificate of Analysis

Download

MSDS

Safety Data Sheet

Download

Request a Document

Protocol Information

Find More Protocols