Hoechst 33342

SKU: orb1301442

Description

Hoechst 33342

Research Area

Cell Biology

Images & Validation

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Item 1 of 1

Key Properties

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CAS Number	23491-52-3
MW	452.55
Purity	99.75%
Formula	C₂₇H₂₈N₆O
SMILES	CCOc1ccc(cc1)-c1nc2ccc(cc2[nH]1)-c1nc2ccc(cc2[nH]1)N1CCN(C)CC1
Target	Autophagy
Solubility	DMSO:41.7 mg/mL (92.14 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (1.1 mM)

Bioactivity

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Target IC50

In Vitro

1. Preparation of Hoechst 33342 working solution 1. Preparation of stock solution Please use DMSO to prepare 1 mg mL stock solution of Hoechst 33342. Note: Hoechst 33342 stock solution is recommended to be stored at -4℃ or -20℃ in the dark after aliquoting. Please avoid repeated freezing and thawing. 2. Preparation of working solution Dilut the stock solution with pure DMEM or PBS to a final concentration of 10 μg mL Hoechst 33342 working solution. Note: Please adjus the concentration of Hoechst 33342 working solution according to actual conditions. It is recommended that you prepare it for immediate use. 2. Cell staining 1. Suspended cells 1) Collect cells by centrifugation and add PBS to was the cells twice. Wash gently for 5 min Each time the recommended cell density is × 06 mL 2) Add 1 mL Hoechst 33342 working solution and incubate at room temperature for 3-10 min. 3) Please use a centrifuge, 400 g, centrifuge for 3-4 min, and discar the supernatant. 4) Add PBS to was the cells twice, gently, 5 min Each time. 5) After resuspendin the cells with 1 mL of pure DMEM or PBS, observe using a fluorescence microscope or flow cytometer. 2. Adherent cells 1) Cultur the adherent cells on a sterile coverslip. 2) Remov the coverslip fro the culture medium and aspirat the excess culture medium, gently. 3) Add 100 μL of Hoechst 33342 working solution, gently shake it to completely cove the cells, and incubate at room temperature for 3-10 min. 4) Aspirat the Hoechst 33342 working solution, wash 2-3 times with pure DMEM, 5 min Each time, and observe using a fluorescence microscope or flow cytometer. Notes： 1. You can adjus the working solution concentration of Hoechst 33342 according to your Experimental purpose. 2. This product is limited to scientific research and is prohibited from being used for Other purposes. 3. For your safety and health, please wear a lab coat and disposable gloves when operating.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNA Stain, Dye reagent, Autophagy, bisBenzimide H 33342, bisBenzimide H33342, bisBenzimide H-33342, inhibit, Inhibitor, HOE33342, HOE-33342, HOE 33342, Hoechst 33342, Hoechst33342, Hoechst-33342

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Chemical structure of Hoechst 33342

Compound Identifiers

PubChem CID

1464 ↗

Key Properties

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No computed properties available.

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Hoechst 33342 (orb1301442)

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Purity:	98.73%
CAS Number:	875756-97-1
MW:	561.93
Formula:	C₂₇H₃₁Cl₃N₆O

Purity:	>98% (HPLC)
CAS Number:	875756-97-1
MW:	561.9
Formula:	C₂₇H₃₁Cl₃N₆O

Hoechst 33342

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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