HP Antibody (Center)

SKU: orb1928202

This product can be custom made

Description

Research Area

Cardiovascular Research, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

−

Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:8000
Reactivity	Human, Mouse

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	45205 Da
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2).

HP Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-HP Antibody (Center) at 1:8000 dilution + Human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45, 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining HP in human epatocarcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

UniProt

P00738

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

Flow Cytometry

View Protocol

Find More Protocols