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Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:100-2000 |
| Reactivity | Human |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,K |
| Clone No. | B423EV0X4X5 |
| Immunogen | This HSPA1A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 574-600 amino acids from human HSPA1A. Antigen Region: 574-600 aa. |
| Target | HSPA1A |
| Molecular Weight | 70052 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
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Confocal immunofluorescent analysis of HSPA1A Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
HSPA1A antibody western blot analysis in HL-60 cell line lysates (35 μg/lane). This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005337.2, NP_005336.3 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
HSPA1A Antibody (orb1939386)
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