HSV-1/HSV-2-IN-3

HSV-1/HSV-2-IN-3 inhibits the helicase-primase complex of herpes simplex virus (HSV), interfering with the crucial processes of DNA unwinding and primer synthesis necessary for viral genome replication. In gD immunofluorescence assays containing 2% FBS, its EC50 against HSV-2 is 7.0 nM, while in assays with 10% human serum, the EC50 is 57.5 nM. Within qPCR replication analysis, the compound demonstrates an EC50 of 1.1 nM. Additionally, HSV-1/HSV-2-IN-3 exhibits strong selectivity against off-target interactions with human carbonic anhydrase (IC50 for hCA II ≈ 2.9 µM, and > 35 µM for hCA I). This compound is applicable in anti-HSV research.