Human 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

SKU: orb782419

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3).

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	3.13-200 ng/mL
Sensitivity	0.88 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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25-HVD3 ELISA Kit, 25HVD3 ELISA Kit, Calcifediol ELISA Kit, Calcidiol ELISA Kit, H-VD3 ELISA Kit, 25-Hydroxycholecalciferol ELISA Kit, 25-Hydroxy Vitamin D3 ELISA Kit, 25(OH)D ELISA Kit

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Human 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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