Human 5-Hydroxytryptamine (5-HT) ELISA Kit

SKU: orb782318

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT).

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Tested Applications

ELISA

Application Notes

standard: 900 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates
Assay Type	Competitive
Assay Time	2.5h
Range	14.07-900 ng/mL
Sensitivity	4.05 ng/mL
Concentration	900 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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5HT ELISA Kit, Serotonin ELISA Kit, N-Methyl-Gamma ELISA Kit

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Human 5-Hydroxytryptamine (5-HT) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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