Human Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit

SKU: orb778615

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 2, Smooth Muscle(ACTa2).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTa2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTa2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 ng/mL
Sensitivity	2.8 ng/mL
Concentration	500 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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α-SMA,ACT-A2 ELISA Kit, ACTSA ELISA Kit, ACTVS ELISA Kit, Asma ELISA Kit, GIG46 ELISA Kit, Cell growth-inhibiting gene 46 protein ELISA Kit

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Human Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit

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UniProt

P62736

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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