Human ALCAM protein

SKU: orb706847

Description

Recombinant Human CD166 Protein is produced by mammalian expression system and the target gene encoding Trp28-Ala526 is expressed with a Fc tag at the C-terminus.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Human
Protein Sequence	WYTVNSAYGD TIIIPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLNLSENYT LSISNARISD EKRFVCMLVT EDNVFEAPTI VKVFKQPSKP EIVSKALFLE TEQLKKLGDC ISEDSYPDGN ITWYRNGKVL HPLEGAVVII FKKEMDPVTQ LYTMTSTLEY KTTKADIQMP FTCSVTYYGP SGQKTIHSEQ AVFDIYYPTE QVTIQVLPPK NAIKEGDNIT LKCLGNGNPP PEEFLFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KKSMIASTAI TVHYLDLSLN PSGEVTRQIG DALPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIQW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADEISDE NREKVNDQAD IEGRMDPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166

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Quick Database Links

UniProt

Q13740

NCBI Gene ID

214

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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