Human Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit

SKU: orb775622

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Intestinal(ALPI).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALPI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.56 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CIP ELISA Kit, IAP ELISA Kit, Calf Intestinal Alkaline Phosphatase ELISA Kit

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Human Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit

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UniProt

P09923

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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