Human Alkaline Phosphatase, Placental (ALPP) ELISA Kit

SKU: orb775674

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Placental(ALPP).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALPP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	125-8000 pg/mL
Sensitivity	50 pg/mL
Concentration	8000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PLAP1 ELISA Kit, ALP ELISA Kit, ALPG ELISA Kit, ALPPL ELISA Kit, GCAP ELISA Kit, Alkaline phosphatase Regan isozyme ELISA Kit, Placental alkaline phosphatase 1 ELISA Kit

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Human Alkaline Phosphatase, Placental (ALPP) ELISA Kit

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UniProt

P05187

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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