Human Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit

SKU: orb1807124

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Description

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-Ⅱ. During the reaction, Human Ang-Ⅱ in the sample or standard competes with a fixed amount of Human Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Competitive
Assay Time	1.5H
Range	31.25-2000pg/mL
Sensitivity	13.7 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Angiotensin-2

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Quick Database Links

Gene Symbol

AngII

UniProt

P01019

UniProt Details

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No UniProt data available

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