Human Anti-HV (Hanta Virus) IgG ELISA Kit

SKU: orb3162828

Description

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-HV IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-HV IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples.
Assay Type	Indirect
Assay Time	3 hours
Range	0.781-50 ng/ml
Sensitivity	0.469 ng/ml

Storage & Handling

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Storage	2-8°C (Sealed), don't cryopreserve.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HV-IgG ELISA Kit, Hanta Virus(HV) IgG ELISA Kit

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Human Anti-HV (Hanta Virus) IgG ELISA Kit

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