Human Anti-Mullerian Hormone (AMH) ELISA Kit

SKU: orb776200

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Mullerian Hormone(AMH).

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Tested Applications

ELISA

Application Notes

standard: 150 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AMH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AMH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	2.35-150 ng/mL
Sensitivity	0.87 ng/mL
Concentration	150 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MIF ELISA Kit, MIH ELISA Kit, MIS ELISA Kit, Müllerian Inhibiting Factor ELISA Kit, Müllerian Inhibiting Hormone ELISA Kit, Müllerian Inhibiting Substance ELISA Kit

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Human Anti-Mullerian Hormone (AMH) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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