Human Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) ELISA Kit

SKU: orb781919

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Proteolipid Protein 1, Myelin Antibody(Anti-PLP1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Proteolipid Protein 1, Myelin Antibody(Anti-PLP1).

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-PLP1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-PLP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-PLP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Competitive
Assay Time	2.5h
Range	3.13-200 ng/mL
Sensitivity	1.17 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MMPL ELISA Kit, PLP/DM20 ELISA Kit, PMD ELISA Kit, SPG2 ELISA Kit, Pelizaeus-Merzbacher Disease ELISA Kit, Spastic Paraplegia 2,Uncomplicated ELISA Kit, Myelin proteolipid protein ELISA Kit, Lipophilin ELISA Kit

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Human Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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