Human Apolipoprotein A5 (APOA5) ELISA Kit

SKU: orb776605

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5(APOA5).

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOA5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.25-80 ng/mL
Sensitivity	0.48 ng/mL
Concentration	80 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Apo-A5 ELISA Kit, APOA-V ELISA Kit, APOAV ELISA Kit, RAP3 ELISA Kit, Regeneration-associated protein 3 ELISA Kit

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Human Apolipoprotein A5 (APOA5) ELISA Kit

Quick Database Links

UniProt

Q6Q788

UniProt Details

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No UniProt data available

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Datasheet

Product Information

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Manual

Product Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

Yeva Bareghamyan et al. Integrated analysis of the blood metallome and DNA methylation, gene expression, and protein levels of immune-associated genes in residents from mining region Environ Geochem Health, (2026)

PubMed: 41485189

Applications

ELISA

Reactivity

Human