Human Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU: orb779280

Featured

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Atrial Natriuretic Peptide(ANP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Atrial Natriuretic Peptide(ANP).

Research Area

Cardiovascular Research, Metabolism Research

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	15.63-1000 pg/mL
Sensitivity	4.66 pg/mL
Concentration	1000 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

ANH ELISA Kit, ANF ELISA Kit, CDD ELISA Kit, Atrial Natriuretic Factor ELISA Kit, Atrial Natriuretic Hormone ELISA Kit, Atriopeptin ELISA Kit, Cardionatrine ELISA Kit, Cardiodilatin ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Human Atrial Natriuretic Peptide (ANP) ELISA Kit

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols