Human Basic Salivary Proline Rich Protein 1 (PRB1) ELISA Kit

SKU: orb777400

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Salivary Proline Rich Protein 1(PRB1).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRB1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	saliva and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.6 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PM ELISA Kit, PMF ELISA Kit, PMS ELISA Kit, PRB1L ELISA Kit, PRB1M ELISA Kit, Ps 1 ELISA Kit, Ps 2 ELISA Kit, PRPPRB1 ELISA Kit, Ps ELISA Kit, cP7 ELISA Kit, Proline-Rich Protein BstNI Subfamily 1 ELISA Kit

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Human Basic Salivary Proline Rich Protein 1 (PRB1) ELISA Kit

Quick Database Links

UniProt

P04280

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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