Human Bone Sialoprotein (BSP) ELISA Kit

SKU: orb775625

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Sialoprotein(BSP).

Research Area

Metabolism Research, Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BSP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	125-8000 pg/mL
Sensitivity	53 pg/mL
Concentration	8000 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

IBSP ELISA Kit, Bsp ELISA Kit, BspII ELISA Kit, SpII ELISA Kit, Integrin-Binding Bone Sialoprotein ELISA Kit, Cell-binding sialoprotein ELISA Kit, Bone sialoprotein 2 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P21815

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols