Human Breast Cancer Susceptibility Protein 1 (BRCA1) ELISA Kit

SKU: orb775768

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 1(BRCA1).

Research Area

Cancer Biology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.067 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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BRCAI ELISA Kit, BRCC1 ELISA Kit, IRIS ELISA Kit, PSCP ELISA Kit, RNF53 ELISA Kit, Breast Cancer 1,Early Onset ELISA Kit, RING finger protein 53 ELISA Kit

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Human Breast Cancer Susceptibility Protein 1 (BRCA1) ELISA Kit

Quick Database Links

UniProt

P38398

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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