Human CD3E & CD3D Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

SKU: orb762434

Description

Human CD3E & CD3D Heterodimer Protein is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).

Research Area

Disease Biomarkers

Images & Validation

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Application Notes

Human CD3E&CD3D Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.2 kDa (CD3E) and 37.3 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 45-60 kDa under reducing (R) condition, and 100-116 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

Key Properties

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Expression System	HEK293
Target	CD3E & CD3D
Reactivity	Human
Tag	C-Fc & C-10×His \| C-Fc & C-Flag
Molecular Weight	40.2 kDa & 37.3 kDa
Expression Region	Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

CD3E & CD3D

UniProt

P07766, P04234

RefSeq:CD3, P07766, P04234

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	CD3, P07766, P04234

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