Human CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

SKU: orb348813

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Description

Human CD3E & CD3G Heterodimer Protein, Fc, His Tag & Fc, Flag Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).

Research Area

Disease Biomarkers

Images & Validation

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Application Notes

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

Key Properties

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Expression System	HEK293
Target	CD3E & CD3G
Reactivity	Human
Tag	C-Fc & C-6×His \| C-Fc & C-Flag
Molecular Weight	39.2 kDa (CD3E) & 38.6 kDa (CD3G)
Expression Region	Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Human CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

SDS-PAGE analysis of Human CD3 epsilon & CD3 gamma Heterodimer protein

Immobilized OKT3 MAb at 10ug/mL (100 uL/well) can bind Human CD3E & CD3G with a linear range of 0.3-5 ug/mL.

Quick Database Links

Gene Symbol

CD3E & CD3G

UniProt

P07766, P09693

UniProt Details

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No UniProt data available

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