Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

Human CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

SKU: orb348813

Description

Human CD3E & CD3G Heterodimer Protein, Fc, His Tag & Fc, Flag Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

Key Properties

Expression SystemHEK293
TargetCD3E & CD3G
ReactivityHuman
TagC-Fc & C-6×His | C-Fc & C-Flag
Expression RegionAsp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW39.2 kDa (CD3E) & 38.6 kDa (CD3G)
Purity95%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP09693, CD3, P07766

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide