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Human CD47 Protein, mFc-His Tag
SKU: orb689369
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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | CD47 |
| Reactivity | Human |
| Tag | C-Mouse Fc and 6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide. |
| Expression Region | CD47(Gln19-Glu141) CD47(Val198-Ala207) CD47(Ser257-Gly268) mFc(Pro99-Lys330) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD47;MER6;IAP;OA3
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Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind its native ligand Human SIRPα, hFc-His tagged protein orb689370 in a linear range of 3.3-26.37 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) orb689306 in a linear range of 0.061-1.606 ng/ml.
ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 Neutralizing antibody orb1173650 in a linear range of 3.2-80.0 ng/ml.
Quick Database Links
Gene Symbol
CD47
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human CD47 Protein, mFc-His Tag (orb689369)
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