Human CDHH(Cadherin, Heart) Microsample ELISA Kit

SKU: orb2998735

Description

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDHH in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.054 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CDH13; P105; T-cad; Cadherin 13; T-Cadherin; H-Cadherin; Truncated cadherin

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Human CDHH(Cadherin, Heart) Microsample ELISA Kit

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UniProt

P55290

UniProt Details

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No UniProt data available

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