Human Chorionic Gonadotropin Alpha Polypeptide (CGa) ELISA Kit

SKU: orb779289

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Alpha Polypeptide(CGa).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CGa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CGa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CGa in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.054 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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FSHA ELISA Kit, GPHA1 ELISA Kit, GPHa ELISA Kit, HCG ELISA Kit, LHA ELISA Kit, TSHA ELISA Kit, Thyroid Stimulating Hormone Alpha ELISA Kit, Follicle-Stimulating Hormone Alpha Subunit ELISA Kit, Luteinizing hormone alpha chain ELISA Kit, Lutropin alpha chain ELISA Kit

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Human Chorionic Gonadotropin Alpha Polypeptide (CGa) ELISA Kit

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UniProt

P01215

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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