Human COL17(Collagen Type XVII) Microsample ELISA Kit

SKU: orb2998640

Description

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL17 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.1 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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COL17A1; BP180; BPAG2; LAD-1; LABD97; Collagen Alpha-1(XVII)chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; Linear IgA disease antigen 1

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