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Human Collagen Type VI protein
SKU: orb750386
Description
Research Area
Protein Biochemistry
Images & Validation
−Item 1 of 8
| Tested Applications | DOT, ELISA, IHC, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human |
|---|---|
| Biological Origin | Human |
| Target | COL6A1-COL6A3 |
| Purity | Human Collagen type VI has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid |
| Concentration | 1.13 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Type VI collagen protein, collagen 6 protein, human collagen protein, collagen VI protein
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COL6 enhances lung epithelial cell wound repair.(a) Representative images of wound-healing response for 16HBE cells plated on COL6, Matrigel, or COL1. (b-c) Quantitation of wound width at 10 hr post-injury (relative to 0 hr) for 16HBE(b) and NHBE(c) plated on individual matrices, or combinations of matrices. N = 9, ** P < 0.01, *** P < 0.001.
COL6 promotes lung epithelial cell spreading.(a) Representative images of initial, post-adhesion spreading for 16HBE human lung epithelial cells 3 hours after plating cells on COL6, COL1 or Matrigel. Arrows show examples of spread cells. (b-d) Quantitation of the percentage of cells spread 3 hours after plating on different matrices for 16HBE (b), NHBE (c), or PHLE (d) cells. N = 6.* P < 0.05, ** P < 0.01, *** P < 0.001.
COL6-mediated spreading requires ITGB1, not NG2.(a) Representative images of untreated 16HBE cells, and 16HBE treated with anti-Integrin β1 or anti-NG2 antibodies 3 hours after plating on COL6. (b) Quantitation of untreated and anti-ITGB1 (b) or anti-NG2 (c) treated 16HBE spreading 3 hours after plating on COL6, COL1, Matrigel coated wells, and uncoated tissue-culture wells (n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001.
Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n orb750386), B) Bovine/Human Collagen VI (p/n orb750380). Load: 1) 1000 ng, 2) 333.33 ng, 3) 111.11 ng, 4) 37.03 ng, 5) 12.35 ng. Primary Antibody: Rabbit Anti-Collagen VI antibody at 5 µg/ml at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 30 mins.
Fourier-transform infrared (FTIR) spectroscopy. Mean FTIR spectra of the five collagen types. Maximal intensity.
FTIR spectra classification model for the five collagen types. n = 30 spectra/collagen type; four spectral intervals, 1, 700–1, 600 cm−1, 1, 480– 1, 350 cm−1, 1, 300–1, 180 cm−1, and 1, 100–1, 005 cm−1. h intracluster heterogeneity, H intercluster heterogeneity.
SDS-PAGE results of Human Collagen Type VI. Lane: 1) Opal prestain molecular weight marker, 2) blank, 3) Human Collagen Type VI (p/n orb750386) [10 µg]. Coomassie stained.
Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI (p/n orb750386) reduced and boiled, 3) Human Collagen Type VI reduced and not boiled, 5) Opal pre-stained molecular weight marker, 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10 µg. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 1hr.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001839.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Human Collagen Type VI protein (orb750386)
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