Human Connexin 43 (CX43) ELISA Kit

SKU: orb776208

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 43(CX43).

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CX43 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.053 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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ODDD ELISA Kit, GJAL ELISA Kit, GJA1 ELISA Kit, GJ-A1 ELISA Kit, Gap junction 43 kDa heart protein ELISA Kit, Gap Junction Protein Alpha 1 ELISA Kit, Oculodentodigital Dysplasia ELISA Kit, Syndactyly Type III ELISA Kit

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Quick Database Links

UniProt

P17302

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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