Human Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

SKU: orb776995

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokine Receptor Like Factor 1(CRLF1).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRLF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRLF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRLF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRLF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.055 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CISS ELISA Kit, CISS1 ELISA Kit, CLF ELISA Kit, CLF-1 ELISA Kit, NR6 ELISA Kit, Cytokine-like factor 1 ELISA Kit, ZcytoR5 ELISA Kit

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Human Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

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UniProt

O75462

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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