Human Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) ELISA Kit

SKU: orb779212

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 2, Heavy Chain 1(DYNC2H1).

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYNC2H1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYNC2H1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYNC2H1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYNC2H1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.122 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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DHC1b ELISA Kit, DHC2 ELISA Kit, DNCH2 ELISA Kit, DYH1B ELISA Kit, hdhc11 ELISA Kit, Dynein heavy chain isotype 1B ELISA Kit, Dynein heavy chain 11 ELISA Kit, Dynein cytoplasmic heavy chain 2 ELISA Kit, Cytoplasmic dynein 2 heavy chain ELISA Kit

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Human Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) ELISA Kit

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UniProt

Q8NCM8

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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