Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit

SKU: orb775832

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epithelial Cell Adhesion Molecule(EPCAM).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPCAM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	12.1 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CD326 ELISA Kit, TACSTD1 ELISA Kit, EGP ELISA Kit, KSA ELISA Kit, M4S1 ELISA Kit, MIC18 ELISA Kit, TROP1 ELISA Kit, HEGP2 ELISA Kit, ESA ELISA Kit, Epithelial glycoprotein ELISA Kit, Adenocarcinoma-associated antigen ELISA Kit, Tumor Associated Calcium Signal Transducer 1 ELISA Kit

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Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit

Quick Database Links

UniProt

P16422

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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