Human Filamin B Beta (FLNb) ELISA Kit

SKU: orb776920

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin B Beta(FLNb).

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLNb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.2 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ABP-278 ELISA Kit, AOI ELISA Kit, FH1 ELISA Kit, FLN1L ELISA Kit, LRS1 ELISA Kit, SCT ELISA Kit, TABP ELISA Kit, TAP ELISA Kit, Actin Binding Protein 278 ELISA Kit, Actin-binding-like protein ELISA Kit, Thyroid autoantigen ELISA Kit, Filamin-3 ELISA Kit, Truncated actin-binding protein ELISA Kit

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UniProt

O75369

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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