Human G Protein Coupled Receptor 109B (GPR109B) ELISA Kit

SKU: orb778431

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 109B(GPR109B).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR109B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR109B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR109B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPR109B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.062 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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HCAR3 ELISA Kit, NIACR2 ELISA Kit, HM74 ELISA Kit, HCA3 ELISA Kit, HM74B ELISA Kit, PUMAG ELISA Kit, Puma-g ELISA Kit, Niacin Receptor 2 ELISA Kit, Nicotinic acid receptor 2 ELISA Kit, Hydroxycarboxylic acid receptor 3 ELISA Kit

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Human G Protein Coupled Receptor 109B (GPR109B) ELISA Kit

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UniProt

P49019

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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