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Human Glucosidase Alpha, Acid (GaA) ELISA Kit

SKU: orb777549
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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid(GaA).

Research Area

Metabolism Research, Protein Biochemistry

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GaA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GaA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GaA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GaA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.07 ng/mL
Concentration10 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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LYAG ELISA Kit, Acid Alpha-Glucosidase ELISA Kit, Lysosomal Alpha-Glucosidase ELISA Kit, Pompe Disease Glycogen Storage Disease Type II ELISA Kit, Acid Maltase ELISA Kit, Aglucosidase Alfa ELISA Kit

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Quick Database Links

UniProt
P10253

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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