Human Granzyme B (GZMB) ELISA Kit

SKU: orb775522

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme B(GZMB).

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Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMB in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	2.7 pg/mL
Concentration	500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GZM-B ELISA Kit, HLP ELISA Kit, CTLA1 ELISA Kit, CCPI ELISA Kit, CGL1 ELISA Kit, CSP-B ELISA Kit, CSPB ELISA Kit, CTSGL1 ELISA Kit, SECT ELISA Kit, Granzyme 2 ELISA Kit, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1 ELISA Kit, Fragmentin 2 ELISA Kit, Cytotoxic Serine Protease B ELISA Kit

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UniProt

P10144

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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