Human Heat Shock 70kDa Protein 12B (HSPA12B) ELISA Kit

SKU: orb780927

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 12B(HSPA12B).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPA12B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPA12B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPA12B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPA12B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.125 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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C20orf60 ELISA Kit

Quality Guarantee

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Quick Database Links

UniProt

Q96MM6

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Human
Dynamic Range:	0.31-20ng/mL
Sensitivity:	0.14ng/mL

Reactivity:	Human
Dynamic Range:	0.31-20ng/mL
Sensitivity:	0.1ng/mL

Reactivity:	Human
Dynamic Range:	0.312-20 ng/mL
Sensitivity:	0.128 ng/mL

Reactivity:	Human
Dynamic Range:	0.312-20 ng/mL
Sensitivity:	0.128 ng/mL

Human Heat Shock 70kDa Protein 12B (HSPA12B) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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