Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

SKU: orb775302

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1(HMG1).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	26.9 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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HMGB1 ELISA Kit, HMG3 ELISA Kit, SBP1 ELISA Kit, Sulfoglucuronyl Carbohydrate Binding Protein ELISA Kit, Amphoterin ELISA Kit, High Mobility Group Box 1 Protein ELISA Kit

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Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

Quick Database Links

UniProt

P09429

UniProt Details

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No UniProt data available

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Datasheet

Product Information

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Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

Mahdieh Azizi 1, Alireza Andalib 2, Marzieh Rezaei Investigating the potential role of miR-451a as a tumor suppressor in immunogenic cell death induction and dendritic cell maturation in colorectal cancer cell lines Cancer Cell Int ., (2025)

PubMed: 40770807

Applications

ELISA

Reactivity

Human