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Human HMGB1 Protein, His Tag
SKU: orb257546
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Description
Images & Validation
−Item 1 of 1
| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | HMGB1 |
| Reactivity | Human |
| Tag | C-10×His |
| Molecular Weight | 26.8 kDa |
| Expression Region | Met 1 - Glu 215 |
| Purity | 90% |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
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−- Item 1 of 1
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 25.7 kDa after removal of the signal peptide. The apparent molecular mass of HMGB1-His is approximately 15-35 kDa due to glycosylation.
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Recombinant Human HMGB1 Protein, His Tag [orb1550480]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Recombinant Human HMGB1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu215) of Human HMGB1 fused with a His Tag at the C-terminal.
100 μgRecombinant Human HMGB1 Protein, N-His [orb2836537]
>90% as determined by SDS-PAGE.
26.97 kDa
50 μg, 20 μg, 100 μgRecombinant Human HMGB1 Protein, N-His [orb2836538]
>90% as determined by SDS-PAGE.
27.2 kDa
100 μg, 20 μg, 50 μgHuman HMGB1 Protein, His Tag [orb2701650]
>93%, determined by SDS-PAGE
This protein contains the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells.
100 μg, 200 μg
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SDS-PAGE analysis of Human HMGB1 protein
Quick Database Links
Gene Symbol
HMGB1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human HMGB1 Protein, His Tag (orb257546)
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