Human IgG F(ab')2 Antibody Fluorescein Conjugated

SKU: orb346658

Description

Human IgG F(ab')2 antibody (FITC)

Images & Validation

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Tested Applications	FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Human
Application Notes	Anti-Human IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Human IgG F(ab')2 fragment
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-Human IgG F(ab')2 fluorescein Conjugated Antibody, rabbit anti-Human IgG F(ab')2 FITC Conjugated Antibody, rabbit anti-Human IgG Fab2 fragment Antibody fluorescein Conjugation

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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