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Human IL12B Protein, His Tag
Description
Research Area
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | IL12B |
| Reactivity | Mouse |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation. |
| Expression Region | IL12B(Ile23-Ser328) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743293]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.
Mammalian
100 μg, 50 μg, 10 μgHuman IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743335]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation.
E.coli
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Mouse TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.
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UniProt Details
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Protocol Information
Human IL12B Protein, His Tag (orb1290821)
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