Human Interferon Regulatory Factor 4 (IRF4) ELISA Kit

SKU: orb866896

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Description

Sandwich Human IRF4 ELISA Kit

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IRF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IRF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IRF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IRF4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.119 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Interferon regulatory factor 4 ELISA Kit, IRF 4 ELISA Kit, IRF-4 ELISA Kit, Irf4 ELISA Kit, IRF4_HUMAN ELISA Kit, LSIRF ELISA Kit, Lymphocyte specific interferon regulatory factor ELISA Kit, Lymphocyte specific IRF ELISA Kit, Lymphocyte-specific interferon regulatory factor ELISA Kit, Multiple myeloma oncogene 1 ELISA Kit, MUM 1 ELISA Kit, MUM1 ELISA Kit, NF EM5 ELISA Kit, NF-EM5 ELISA Kit, NFEM5 ELISA Kit, PU.1 interaction partner ELISA Kit, Sfpi1/PU.1 interaction partner ELISA Kit, Transcriptional activator PIP ELISA Kit

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Human Interferon Regulatory Factor 4 (IRF4) ELISA Kit

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UniProt

Q15306

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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