Human Interleukin 12 p40 (IL-12p40) ELISA Kit

SKU: orb776127

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B(IL12B).

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	2.8 pg/mL
Concentration	500 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

p40 ELISA Kit, IL12-B ELISA Kit, CLMF2 ELISA Kit, NKSF2 ELISA Kit, Natural Killer Cell Stimulatory Factor 2 ELISA Kit, Cytotoxic Lymphocyte Maturation Factor 2 ELISA Kit, Interleukin 23, P40 Subunit ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Human Interleukin 12 p40 (IL-12p40) ELISA Kit

Quick Database Links

UniProt

P29460

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols