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Human MMP-9 Protein, His Tag

SKU: orb257692

Description

Human MMP-9, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 469 (Accession # AAH06093). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Research Area

Cancer Biology; Musculoskeletal & Connective Tissue Research

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetMMP-9
ReactivityHuman
TagC-6×His
Expression RegionAla 20 - Pro 469
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW50.8 kDa
Purity90%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 150 mM NaCl, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqAAH06093

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide