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Human MMP13 Protein, His Tag (Pro-form, HPLC verified)

SKU: orb3158090

Description

Human MMP13 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Asn 274 (Accession # P45452-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.5 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetMMP13
ReactivityHuman
TagC-10×His
Expression RegionLeu 20 - Asn 274
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW30.5 kDa
Purity95%

Storage & Handling

Storage-70°C
Form/AppearanceLiquid
Buffer/Preservatives50 mM Tris, 200 mM NaCl, pH 8.0
Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only
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NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP45452

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide